Novel Alhagi maurorum leaves mediated synthesis of titanium nanoparticles for human breast carcinoma applications: a preclinical trial study

Abstract

IntroductionIn this study, titanium nanoparticles (TiNPs) were synthesized in an aqueous medium using Alhagi maurorum extract as a stabilizing and reducing agent.Material and methodsUltraviolet–visible spectrophotometry (UV-Vis), Fou�rier-transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and energy dispersive X-ray spectrometry (EDS) were the techniques to characterize the biosynthesis of TiNPs. To survey the anti-human breast cancer effects of TiNPs, MTT assay was used on the common breast cancer cell lines, i.e., breast cancer (breast adenocarcinoma (MCF7), infiltrating ductal cell carcinoma (Hs 319.T), inflammatory carcino�ma of the breast (UACC-732), and metastatic carcinoma (MDA-MB-453)) cell lines. TiNPs’ crystal size was measured as 16.08 nm. SEM images exhibited a uniform spherical morphology in size range of 12.16 to 43.46 nm for the biosynthesized nanoparticles respectively.ResultsThe cell viability of breast carcinoma cells decreased dose-de�pendently in the titanium nanoparticles’ presence. The IC50 values of A. mau�rorum and titanium particles on the MCF7 cell line were 680 and 359 µg/ml, on the Hs 319.T cell line were 507 and 191 µg/ml, on the UACC-732 cell line were 477 and 217 µg/ml, and on the MDA-MB-453 cell line were 507 and 191 µg/ml, respectively. TiNPs had high anti-breast cancer activities dose-dependently against MCF7, Hs 319.T, UACC-732, and MDA-MB-453 cell lines.ConclusionsThe best result of anti-breast cancer effects was seen in the case of the Hs 319.T cell line.