Abstract
Acinetobacter baumannii appears to be one of the most crucial nosocomial pathogens. A possible component of antimicrobial therapy for infections caused by extremely drug-resistant A. baumannii strains may be specific lytic bacteriophages or phage-derived enzymes. In the present study, we observe the biological features, genomic organization, and phage–host interaction strategy of novel virulent bacteriophage Aristophanes isolated on A. baumannii strain having K26 capsular polysaccharide structure. According to phylogenetic analysis phage Aristophanes can be classified as a representative of a new distinct genus of the subfamily Beijerinckvirinae of the family Autographiviridae. This is the first reported A. baumannii phage carrying tailspike deacetylase, which caused O-acetylation of one of the K26 sugar residues.
Highlights
Nowadays, the ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp.)are the leading cause of nosocomial infections worldwide [1]
A. baumannii strains can produce a vast variety of capsular polysaccharides (CPSs) which serve as primary receptors for the majority of phages that carry genes encoding polysaccharide-degrading enzymes [4,5,6,7,8,9,10,11]
The A. baumannii phage Aristophanes was isolated from a sewage sample and was found to be infectious to A. baumannii strain KZ1098 belonging to K26 capsular type
Summary
The ESKAPE pathogens (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter sp.)are the leading cause of nosocomial infections worldwide [1]. In 2017, carbapenem-resistant A. baumannii strains was designated by the World. A possible component of antimicrobial therapy of infections caused by drug-resistant A. baumannii strains can be specific lytic bacteriophages or enzymes and antibacterial proteins encoded in their genomes. A. baumannii strains can produce a vast variety of capsular polysaccharides (CPSs) which serve as primary receptors for the majority of phages that carry genes encoding polysaccharide-degrading enzymes [4,5,6,7,8,9,10,11]. There are descriptions of depolymerase-carrying bacteriophages infecting A. baumannii strains belonging to 15 various K types [4,5,7,8,9,10,11,12]
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