Abstract
Increasing studies have suggested that dysregulation of RNA‐binding proteins (RBPs) contributes to cancer progression. Neuro‐oncological ventral antigen 1 (NOVA1) is a novel RBP and plays an important role in tumour development. However, the expression and role of NOVA1 in melanoma remain unknown. In this study, we indicated that NOVA1 expression was up‐regulated in melanoma samples and cell lines. Moreover, we demonstrated that knockdown of NOVA1 suppressed melanoma cell proliferation, migration and invasion in both A375 and A875 cell lines. In addition, we showed that suppressed expression of NOVA1 enhanced forkhead box O3a (FOXO3a) expression while inhibited AKT expression in melanoma cell. Furthermore, we demonstrated that inhibited expression of FoxO3A rescued NOVA1‐mediated cell proliferation, migration and invasion in melanoma cell line A375. These results suggested that NOVA1 acted as an oncogene in the development of melanoma partly through regulating FoxO3A expression.
Highlights
Melanoma is derived from melanocytes and responsible for most of skin tumour-related deaths in human beings.[1,2,3,4,5] There are about 76 380 new patients of melanoma and approximately 10 130 melanoma-related deaths in 2016 in the United States.[6]
We demonstrated that knockdown of Neuro-oncological ventral antigen 1 (NOVA1) suppressed melanoma cell proliferation, migration and invasion in both A375 and A875 cells
We showed that suppressed expression of NOVA1 enhanced FoxO3A expression and suppressed AKT expression in melanoma cell line A375
Summary
Melanoma is derived from melanocytes and responsible for most of skin tumour-related deaths in human beings.[1,2,3,4,5] There are about 76 380 new patients of melanoma and approximately 10 130 melanoma-related deaths in 2016 in the United States.[6]. Increasing studies have showed that RNA-binding proteins (RBPs) are important for post-transcriptional regulation.[13,14,15,16] Several studies have investigated the roles of RBPs in the prognosis and progression of melanoma.[17,18,19] Neuro-oncological ventral antigen 1 (NOVA1) is one member of RBPs and is involved in the programme of neural splicing.[20,21] The NOVA superfamily has two members such as NOVA1 and NOVA2. We explored the potential mechanism of NOVA1 in melanoma.
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