Notice of Retraction: Quantification of PAHs Pollution in Marine Environment by Measurements of EROD Activity and Caspase-3/7 Activity in Marine Madaka (Oryzias melastigma) Embryos

Abstract

Marine model fish medaka and their embryos can be used as an ideal research materials in the field of marine ecotoxicology due to their highly sensitive to various environmental pollutants. In this study, we took marine medaka (Oryzias melastigma) and their embryos as subject materials, and used the activities of embryonic EROD (7-ethoxyresorufin-O-deethylase) and Caspase-3/7 as biomarkers to study the typical polycyclic aromatic hydrocarbon (PAH) such as benzo (a) pyrene, phenanthrene and pyrene induced the toxicity and dose-response relationship of marine fish. The results showed that both of EROD activity and Caspase-3/7 activity in embryos could be induced by benzo(a)pyrene, phenanthrene and pyrene significantly. In particular, the significant induction of EROD activity by specific PAHs can be used for early warning and PAHs detection in the marine environment. The Caspase-3/7 in the fertilized eggs(1-3 dpf) can also be significantly induced by PAHs which can be used to monitor PAHs pollution as another promising biomarkers. In general, these methods to quantify and assess PAHs pollution in the marine environment are sensitive and have good application prospects.