Not only greening: The effects of marennine produced by Haslea ostrearia on physiological traits of three bivalve species

Abstract

The marine diatom Haslea ostrearia is known for its ability to produce marennine, a blue-green water-soluble pigment, which is responsible for the oyster greening phenomenon in the French Atlantic Coast. Marennine can be considered a bioactive extracellular compound (BEC) as it exhibits activities against detrimental pathogens in bivalve aquaculture. However, marennine could also be toxic to animals. The aim of this experimental study was to assess the effect of marennine produced by H. ostrearia upon three commercially important bivalve species, the American oyster Crassostrea virginica, the blue mussel Mytilus edulis and the giant scallop Placopecten magellanicus. Effect of this BEC was also assessed on different ontogenic stages of scallops. The animals were exposed to 3 mg L−1 of marennine and physiological response associated with clearance rates (CR), oxygen consumption and condition index (CI) were measured after 16 h of exposure (day 0), and after one subsequent week of recovery (day 7). In the short term, CR and oxygen consumption on mussels and oysters were significantly altered by marennine but not for scallops. CR recovery was observed in oysters after seven days, but no depuration was observed in mussels. Nevertheless, oxygen consumption in both mussels and oysters recovered on day 7. CI in the marennine-exposed group for both adult oysters and scallops was not significantly different, yet CI of mussels was lowered by 18.2 % compared to control. Scallop adults seemed largely resistant to marennine, but juveniles were more susceptible with a reduced CR and oxygen consumption by 71.9 % and 24.5 %, respectively, and no depuration activity observed until day 7. Additionally, CI in marennine-exposed juvenile scallops was 27 % lower than that of control. This study demonstrates species- and age-specific effect of marennine on bivalves, thus highlighting the importance of further assessment on the utilization of this pigment as a natural product decreasing pathogenicity of bacteria in shellfish aquaculture systems.