Abstract
Overconsumption of abnormally elevated blood metabolites by erythrocyte-encapsulated enzyme proteins has beeen shown to be feasible.1,2 Along this line we examined the possibility of using red blood cells (RBC) loaded with glucose catabolizing enzymes to regulate blood glucose around physiological levels in the hyperglycemic state. In previous studies3 we reported the biochemical properties of human and mice erythrocytes upon encapsulation of glucose oxidase (GOD) alone or coentrapped with hexokinase (HK). The “in vitro” results showed that the cells had an enhanced glucose consumption and acceptable biological properties. In order to establish their use as potential bioreactors for control of hyperglycemias, “in vivo” studies were performed. The results reported in this paper show that erythrocytes glucose oxidase and hexokinase-loaded have a slightly decreased survival and are able to regulate blood glucose around physiological levels in diabetic mice. These results suggest that enzyme-loaded erythrocytes can behave as circulating bioreactors and can provide a new way for the overconsumption of abnormally elevated blood glucose.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
