Normality of The Mice's Fetal Spine during Fennel Flower Extract (Nigella sativa) Feeding

Abstract

Black cumin (Nigella sativa ) is an annual flowering herbal plant that is widely planted in India, Egypt, and the Middle East. Black cumin essential oil contains p-cymene, α-pinene, dithymoquine, thymohydroquinone, and thymoquinone (more than 50%). This study aims to determine the effect of black cumin extract on the structure of the spine, length, and fetal weight of the mouse. The study was conducted in December 2019 to February 2020 at the Zoology and Botany Laboratory of the Faculty of Mathematics and Natural Sciences, Universitas Lampung. This study uses a Completely Randomized Design (CRD) consisting of 4 groups, each group with 5 repetitions (K1, P1, P2, P3). Black cumin extract is given orally as much as 0.3 ml/day on the 6th to 17th day of pregnancy. The control group K1 (Aquabides), P1 was given black cumin extract (2.1 mg/30 g), P2 (8.4 mg/30 g), and P3 (33.6 mg/30 g). Data on the structure of the spine were observed descriptively while the length and weight of the fetus of mice were analyzed using ANOVA (Analysis of Variance) with a significance level of α = 5%. The results obtained on the structure of the spine from each treatment did not cause abnormalities in the structure of the mice's fetal spine. Black cumin contains calcium, magnesium, potassium, sodium, and iron which are useful in the process of bone growth and development. Besides, the results of fetal length and weight data based on the ANOVA test one factor level α = 5% statistically did not show a decrease in the length and weight of the fetus of mice.