Abstract
If normal mouse thymus or lymph node cells are cultured in the agar medium normally used for growing bone marrow colonies, death of the cells occurs very rapidly. Most cells reharvested after 2–3 h are stainable by eosin and by 24 h, the only surviving cells are occasional macrophages. From 1966 onward, many attempts were made to obtain survival and proliferation of T- or B-lymphocytes in agar cultures, but these were unsuccessful, despite the use of a variety of conditioned media or underlayers.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.