Abstract

If normal mouse thymus or lymph node cells are cultured in the agar medium normally used for growing bone marrow colonies, death of the cells occurs very rapidly. Most cells reharvested after 2–3 h are stainable by eosin and by 24 h, the only surviving cells are occasional macrophages. From 1966 onward, many attempts were made to obtain survival and proliferation of T- or B-lymphocytes in agar cultures, but these were unsuccessful, despite the use of a variety of conditioned media or underlayers.

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