NORMAL INDICATORS OF OXIDATIVE STRESS AND THE DYNAMICS OF THEIR CHANGES AT DIFFERENT TERMS OF EXPERIMENTAL OPIOID INFLUENCE

Abstract

In the modern world, there are a large number of exogenous factors that contribute to the emergence of disorders associated with changes in biochemical data. One of these factors is the long-term usage of the opioid analgesics for therapeutic purposes, as well, as the use of opioid drugs without medical indications by people belonging to the category of drug addicts. More often, doctors of various specialties are faced with the problem of treating various nosology in people with opioid addiction. The process of correction of the main pathology makes it diffi cult due to the consequences arising from the chronic use of opioid drugs. The aim of the study is to fi nd out the dynamics of changes in the oxidative stress indicators of the blood of rats under normal conditions and during subchronic and chronic experimental opioid exposure. Material and methods. The research material was sexually mature, purebred male rats in the amount of 38 animals, weighing 160.0-270.0 g, aged 4.5-7.5 months. The animals were injected with nalbuphine intramuscularly, once a day at one time (10-11 am) per 70 days. The initial dose of nalbuphine during the fi rst 2 weeks was 0.212 mg/kg, the next 2 weeks (II-IV weeks) – 0.225 mg/kg, the next (IV-VI weeks) – 0.252 mg/kg, the next (VI-VIII weeks) – 0.260 mg/kg, and during (VIII-X weeks) – 0.283 mg/kg. Thus, conditions for chronic opioid exposure were created. Research results and their discussion. As a result of our experimental study, the eff ect of an opioid analgesic on the indicators of oxidative stress in the blood of rats, at the end of the 2nd week, we observed an unreliable increase in malondialdehyde and diene conjugates with a signifi cant decrease in the ceruloplasmin indicator. At the end of the 6th week administration of opioid analgesics, we observed a steady and continued growth of malondialdehyde and diene conjugates and a further decrease in ceruloplasmin. All indicators had a statistically proven diff erence compared with the control group. The animals of the experimental group, after 10 weeks of experimental opioid exposure have shown that all indicators continued the previously identifi ed dynamics and reached their maximum value of the diff erence compared to the control group. Conclusions. The conducted studies make it possible to clarify the peculiarities of changes in the oxidative stress indicators of the blood of rats in the subchronic and chronic periods of experimental opioid exposure.