Abstract
"Monocytes are leukocytes that can differentiate into tissue macrophages and act in immune surveillance and tissue reconstruction. They are frequently used in vitro to study inflammatory response, lymphocyte activation, and phagocytosis. A widely used model is the in vitro differentiation of various monocytic cell lines including THP-1 and U937 using phorbol 12- myristate 13-acetate (PMA). These cell lines have various limitations because of their malignant background. Data available on utilizing normal human monocytes cell lines are scarce. Our study aims to identify the optimum concentration of PMA needed to maximize the number of adherent macrophages using the normal monocytic cell line CRL-9855SC (ATCC CRL-9855). Using impedance readings and time-lapse microscopy, we determined that the optimum PMA concentration is 25ng/mL for 48 hourshrs. Using these optimized conditions, we were able to induce the formation of adherent and proliferating macrophages, which can be further used for morphology and functional studies."
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