Normal Human Immune Peritoneal Cells: Phenotypic Characteristics

Abstract

Normal human peritoneal cells (PC) collected from patients with calculous cholecystitis without clinically detectable inflammatory changes were characterized morphologically, histochemically and phenotypically by means of monoclonal antibodies. The PC consisted of 45 % of monocytes/macrophages (M718+ cells). Thirty-five per cent of PC were esterase-positive and 23 % acid phosphatase positive. Forty-five per cent of PC adhered to glass surface. In the lymphocyte population, 2 % of CD22 B lymphocytes (M738+) and 42 % CD2 T lymphocytes (M720+) were found. CD4/CD8 ratio was 0.4. There were 8 % of Leu7+ cells. The PC did not reveal interleukin 2 (OKT26a+) and transferrin receptors (OKT9+) on their surface. No blast cells were detected in the PC suspension. Approximately 49 % of the PC expressed la antigens (OKIa1 +). Two per cent of S100 positive dendritic cells (2311 +) were found. Peritoneal fluid contained 9 % of granulocytes, mostly neutrophils. Two per cent of PC were free mesothelial cells (M717+). We conclude that human peritoneal cavity contains a cell population significantly differing from that which is present in peripheral blood, which strongly suggests a non-random cell accumulation in the peritoneum. Lack of any activated cells indicates that under normal conditions the peritoneal lavage fluid contains a steady-state population. We conclude that the normal peritoneal fluid cells represent a heterogeneous population capable of reacting to various antigens entering the cavity from the gut.