Abstract
Surface preparations of the organ of Corti, dissected from cochleas fixed and stained with OsO4, or from fresh specimens, can be examined immediately by phase-contrast microscopy, according to the method of Engström et al. [Acta Oto-Laryngol. Suppl. 188, 92–99 (1964)] without the delays and distortions occasioned by decalcification and celloidin embedding. Since both the cytoarchitectural pattern and the condition of individual cells throughout the entire length of Corti's organ can be studied directly and in detail, the method provides information that cannot be obtained from the familiar stained serial sections of temporal bones. Photomicrographs are presented to illustrate the method of preparation and the appearance of the organ of Corti, the stria vascularis, and the basilar, tectorial, and Reissner membranes of the normal guinea pig.
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