Normal B cell development and Pax5 expression in Thy28/ThyN1-deficient mice.

Fusako Kitaura,Toshitsugu Fujita,Hodaka Fujii,Kazuo Yamagata,Jun Ueda,Miyuki Yuno,Shigeharu Wakana,Sebastian D Fugmann

doi:10.1371/journal.pone.0220199

Abstract

Thy28, also known as ThyN1, is a highly conserved nuclear protein. We previously showed that in a chicken mature B cell line, Thy28 binds to the promoter of the gene encoding Pax5, a transcription factor essential for B cell development, and positively regulates its expression. Here, we generated a Thy28-deficient mouse line to analyze its potential role in B cell development in mice. Thy28-deficient mice showed normal development of B cells, and the expression of Pax5 was comparable between wild-type and Thy28-deficient primary B cells. Thus, species-specific mechanisms regulate Pax5 expression and B cell development.

Highlights

B cell development is a complex process regulated by the concerted actions of many gene products
We found that Thy28, which is known as ThyN1, binds to the promoter region of the Pax5 gene in a B cell-specific manner and positively regulates its expression [6]
These results indicated that our targeting strategy effectively knocked out the Thy28 gene in these mice

Summary

Introduction

B cell development is a complex process regulated by the concerted actions of many gene products. Expression of the mouse Pax gene is regulated by many transcription factors and DNA-binding proteins. Examples of such regulators include PU., IRF4, IRF8, NF-κB, and EBF1 [2, 3]. We previously used a locus-specific chromatin immunoprecipitation (ChIP) approach to analyze the mechanisms regulating the expression of Pax in a chicken mature B cell line, DT40 [4][5]. We found that Thy, which is known as ThyN1, binds to the promoter region of the Pax gene in a B cell-specific manner and positively regulates its expression [6]

Methods

Results

Conclusion