Normal and Reversed Phases Thin-Layer Chromatography of Arylidene 2- Thiohydantoin Derivatives

Abstract

In this paper, the retention behavior of thirteen newly synthesized arylidene derivatives of 2-hydamthione was investigated by normal and reversed phases thin-layer chromatography. In normal phase chromatography, thin layers of silica gel and cyano-propyl (CN) silica gel with fluorescent indicator F254 were used as the stationary phase. As the mobile phase, binary systems of non-aqueous solvents benzene-ethyl acetate and hexane-ethyl acetate were used, 8:2 (v/v). Reversed phase chromatography was performed using a thin layer of octadecyl (C-18) silica gel with fluorescent indicator F254 as the stationary phase and a binary solvent mixture of acetonitrile-water in the ratio 7.5:2.5 (v/v) as the mobile phase. Observing under a UV lamp, the spots of all tested derivatives were clearly visible. The RF value was determined for each spot. Based on the obtained results the HPLC technique is recommended for further chromatographic examination of the newly synthesized arylidene derivatives of 2-hydamthione, using commercial columns with polar chemically bound phases and both non-aqueous and aqueous eluents.