Abstract
Tumorous tobacco shoots have been derived from a nopaline-type callus tissue (SR1-4058-38) produced by transformation of tobacco protoplasts via co-cultivation with Agrobacterium tumefaciens. When grafted onto normal tobacco stems the shoots produced flowers that were male sterile. Seeds were obtained from crosses between grafted SR1-4058-38 regenerants and normal tobacco plants. Germination of these seeds on tissue culture medium gave two phenotypically different classes of progeny plants. One half of the seedlings appeared normal, whereas the other half contained the T-DNA specific enzyme nopaline dehydrogenase (NpDH). Thus, this T-DNA marker is transmitted to the F1 progeny plants with Mendelian segregation ratios.
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