Nonylphenol induced individual and population fluctuation of Caenorhabditis elegans: Disturbances on developmental and reproductive system

Abstract

The environmental risks that have arisen from endocrine disruption compounds (EDCs) have become global challenges, especially for persistent bio-accumulated xenobiotic chemicals, such as nonylphenol (NP). In the present study, the population dynamics of Caenorhabditis elegans (C. elegans) were systemically investigated by conducting developmental and reproductive bioassays under the exposure of NP, which has been widely detected in actual aquatic environments. The results revealed that under NP exposure (400 μg L−1 NP), developmental indictors of C. elegans, including the body length and width were significantly inhibited at different life stages of L1 and L4 larva, and the growth curves were further adversely affected. In addition, abnormalities in reproductive systems were also observed under NP exposure. Such abnormalities obeyed a dose-dependent relationship with NP levels, which were closely related to the delayed spawning time and decreased reproductive rates. Moreover, the results from global genome expression analysis for nematodes revealed that the most significant enriched GO terms could be predominantly responsible for the dysregulation of growth and reproductive system. The population's parameters, including age composition and intrinsic growth rate (rm d−1), displayed significant changes, with a suppressed potentiality of population growth. Those data elucidated that NP exhibited a profound impact on the dynamic stability of the population, even with no obvious effect on certain biochemical markers.