Abstract
Among 34 Streptococcus pneumoniae ( Spn) sequential isolates from middle ear fluid, we found a case of a nontypeable S. pneumoniae (NT- Spn) in a child with acute otitis media (AOM). The strain was pneumolysin PCR positive and capsule gene PCR negative. Virulence of the NT- Spn was confirmed in a chinchilla model of AOM.
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