Abstract
BackgroundThe main cause of bovine tuberculosis (bTB) is believed to be Mycobacterium bovis (M. bovis). Nontuberculosis mycobacteria (NTM) are neglected but opportunistic pathogens and obstacles for bTB diagnosis. This study aimed to isolate and characterize the mycobacteria organisms involved in causing TB-like lesions in cattle in northwestern Ethiopia.ResultsA total of 2846 carcasses of cattle were inspected for TB lesions. Ninety six tissues (including lymph nodes such as submandibular, retropharyngeal, tonsilar, mediatinal, bronchial and mesenteric, and organs such as lung, liver and kidney) with suspicious TB lesion(s) were collected and cultured on Lowenstein-Jensen medium. Twenty one showed culture growth, of which only 17 were identified containing acid fast bacilli (AFB) by Ziehl–Neelsen staining. Among the 17 AFB isolates 15 generated a polymerase chain reaction product of 1030 bp by gel electrophoresis based on the 16S ribosomal RNA gene amplification. No M. tuberculosis complex species were isolated. Further characterization by Genotype Mycobacterium CM assay showed 6 isolates identified as M. peregrinum. Eight isolates represented by mixed species, which includes M. fortuitum-peregrinum (3 isolates), M. gordonae-peregrinum (3 isolates) and M. fortuitum-gordonae-peregrinum (2 isolates). One NTM could not be interpreted.ConclusionA significant number of NTM species were isolated from TB-like lesions of grazing cattle slaughtered at Bahir Dar Abattoir. Such finding could suggest the role of NTM in causing lesions in cattle. Further investigations are recommended on the pathogenesis of the reported NTM species in cattle, and if they have public health significance.
Highlights
The main cause of bovine tuberculosis is believed to be Mycobacterium bovis (M. bovis)
Bovine tuberculosis, which is primarily caused by Mycobacterium bovis (M. bovis) is an endemic disease of cattle in Ethiopia and distributed in almost all parts of the country
Eight isolates represented by mixed species such as M. fortuitumperegrinum (3 isolates), M. gordonae-peregrinum (3 isolates) and M. fortuitum-gordonae-peregrinum (2 isolates)
Summary
The main cause of bovine tuberculosis (bTB) is believed to be Mycobacterium bovis (M. bovis). Nontuberculosis mycobacteria (NTM) are neglected but opportunistic pathogens and obstacles for bTB diagnosis. This study aimed to isolate and characterize the mycobacteria organisms involved in causing TB-like lesions in cattle in northwestern Ethiopia. Bovine tuberculosis (bTB), which is primarily caused by Mycobacterium bovis (M. bovis) is an endemic disease of cattle in Ethiopia and distributed in almost all parts of the country. There have been no studies to date conducted to identify the specific species of NTMs that are causing TB lesions in cattle in northwest Ethiopia. Easy and sensitive molecular tools are available for the detection and identification of MTBC and NTM [22]. In the present study NTMs were predominantly isolated and characterized from TB-like lesions of cattle by molecular tools such as mycobacterium genus typing and Genotype Mycobacterium CM assay. One NTM could not be interpreted even if it had a band pattern of 1,2,3 and 10, and no MTBC species were identified
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