Abstract
BackgroundPorcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. Previous studies have indicated that the nonstructural protein 11 (nsp11) of PRRSV may be an important protein for the immune escape of PRRSV.ResultsHere, we firstly explored the effect of over-expression of nsp11 on PRRSV infection and found that over-expression of nsp11 enhanced the PRRSV titers while the small interfering RNA (siRNAs) specifically targeting nsp11 could reduce the PRRSV titers in MARC-145 cells.ConclusionIn conclusion, PRRSV nsp11 promotes PRRSV infection in MARC-145 cells and siRNAs targeting nsp11 may be a potential therapeutic strategy to control PRRSV in future.
Highlights
Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it
Other and our previous work demonstrated that PRRSV nsp11 inhibited the host innate immune responses such as the transcription of type I interferon [7], the RNA interference (RNAi) innate immune response [9] and the NLR family pyrin domain-containing 3 (NLRP3)-mediated production of IL-1β [10], which indicated that PRRSV nsp11 may play an important role in PRRSV infection
The MARC-145 cells were cultured in 24-well plates overnight, and the cells were transfected with the expression plasmid pcDNA3.1-GFP-nsp11 or the control plasmid pcDNA3.1-GFP
Summary
Porcine reproductive and respiratory syndrome virus (PRRSV) induces one of most important devastating disease of swine worldwide, and the current methods poorly control it. PRRSV, a positive sense and single-stranded RNA virus, is a member of family Arteriviridae [1] Since it was emerged in the United States in 1987 and in Europe in 1990, PRRSV has rapidly spread in the swine producing regions and became one of the most important devastating diseases of swine worldwide. It can cause severe reproductive failure in sows and respiratory distress in young growing pigs [2]. Previous studies have shown that the nsp of equine arteritis virus(EAV), which is another member of family Arteriviridae, may play a key role in viral RNA synthesis and additional functions in the viral life cycle [8]. The purpose of present study is to explore the effect of over-expression of nsp on PRRSV infection and whether the siRNAs targeting the PRRSV nsp could influence PRRSV infection
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