Nonrandom ectopic protein production by malignant cells: direct evidence in vitro.

Abstract

A large number of cell lines derived from malignant human neoplasms (21 carcinomas or melanomas and 11 sarcomas or gliomas) and 18 human fibroblast lines were examined for ectopic protein production by RIA. By conservative criteria, 13 malignant lines produced ectopic CG or its s-subunit (0.5–5.6 pmol/mg cell protein). Four of these lines produced ectopiccarcinoembryonic antigen (0.04–0.95), while none produced placental lactogen or ∞-fetoprotein (<0.10). Six malignant lines produced ectopically the common a-subunit of the glyco-protein hormones, and in 2 (ChaGo lung and Chang liver), the secretion rates (391 and 506 pmol/mg cell protein-24 h) were almost 100 times higher than that of any other ectopic protein. Eight malignant lines produced low levels of PRL (0.1–0.57), and 3 lines produced low levels of LH or its ssubunit (0.1–0.26), but neither was secreted. In contrast, only 2 normal fibroblast lines produced CG, and 1 produced PRL-like activity. None of the malignant or fibroblast lines produced GH o...