Abstract

A review of the principles and current status of nonradioisotopic immunoassays in the context of therapeutic drug monitoring application is presented. The methods discussed include 1) light scattering, both nephelometric and particle aggregation; 2) fluorescence; 3) immunoenzyme; and 4) chemiluminescence. Basically, all of these methods have demonstrated acceptable sensitivity and replicability for application to drugs of current interest to therapeutic drug monitoring. Each system presents advantages and disadvantages which must be reviewed by the user before making a decision for individual laboratory application.

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