Abstract
The first non-radioactive digoxigenin labeled cDNA probes were prepared from part of coat protein gene of the American (No.45058, American Type Culture Collection), Czech (A52/E6), German (Chandler/E6) and Norwegian (Mimek/Alpine) isolates of strawberry vein banding virus (SVBV). All five American different air-dried SVBV leaf samples originating from National Clonal Germplasm Repository (N.C.G.R., Corvallis, U.S.A) and six from twelve Czech strawberry isolates exhibiting SVBV-like infection symptoms reacted positively in dot blot hybridization with American probe. Twenty six Czech strawberry SVBV samples were used in dot blot with probes prepared against the Czech, American, German and Norwegian isolates. Our results showed that the samples reacted identically, independently on the origin of the probe. They confirm the spread of SVBV in Central Europe and introduce the first reliable screening method for this virus.
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