Abstract

Dot and slot hybridization is a nucleic acid analysis technique in which an excess of labeled probe is hybridized to a target that has been immobilized on a solid support. Currently nucleic acid hybridizations are most frequently performed by radioactive detection methods particularly by using the 32P-labeling of nucleic acids as the preferred technique [1, 2]. Different nonradioactive methods e.g. using probes labeled with biotin [3] or digoxigenin-dUTP (DIG) [4] have been introduced as possible alternatives to radioactive assays enabling even higher sensitivity than 32P-based hybridizations [4].

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