Non‐peptide Agonist of the Receptor MAS, AVE 0991, Increases PPARα Expression in Muscle of Rats Fed Fructose‐ Rich Diet

Abstract

The aim of the present study was to evaluate the role of AVE 0991, agonist non‐peptide of Angiotensin‐(1–7), in lipidic profile of Sprague‐Dawley male rats. Animals were randomly distributed into fed commercial diet plus 10% fructose in drinking water during 3 weeks, after animals were treated with (FA) or without (F) 1 mg/Kg AVE0991 during 2 weeks. To evaluate the effects of AVE, it was analyzed: serum biochemical parameters, activity of lipoprotein lipase (LPL) in muscle and gene expression of PPARγ, SREBP‐1c and PPARα by real time PCR. Treatment with AVE0991 promoted reduction in serum levels of free fatty acids (F 0.707±0.02mmol/L vs. FA 0.558±0.06mmol/L) and triacylglycerol (F 163.9±18.18mg/dL vs. FA 108.7±11.65mg/dL) without altering the levels of total cholesterol in animals fed with fructose. The activity of LPL in muscle was increased (F 0.014±0.005mU vs. FA 0.04±0.01mU) without changing gene expression of PPARγ and SREBP‐1c. However, there was an increase in gene expression of PPARα (F1.1±0.14 unit arbitrary vs. FA 5.4±1.8 unit arbitrary). These data suggest that treatment with AVE0991 increased the consumption of lipids by muscle, and this can contribute to the reduction of free fatty acids and triacylglycerol found in serum of rats fed a fructose‐rich diet.Financial support: CNPq, CAPES, FAPEMIG.