Nonomuraea lycopersici sp. nov., isolated from the root of tomato plants (Solanum lycopersicum L.).

Abstract

Two novel bacterial strains were isolated from the roots of tomato plants (Solanum lycopersicum L.) from Dengfeng, Henan Province, China. Phylogenetic analysis of 16S rRNA gene sequences showed that the isolates NEAU-DE8(1)T and NEAU-HE1(2) are closely related to one another (99.6% similarity) and are closely relate to Nonomuraea zeae NEAU-ND5T (98.3, 98.3%) and Nonomuraea gerenzanensis DSM 100948T (98.3, 98.0%). Phylogenetic trees constructed based on 16S rRNA gene sequences revealed that both strains grouped with N. zeae NEAU-ND5T within the clade comprising the members of the genus Nonomuraea. Moreover, key chemotaxonomic properties including major menaquinones, fatty acid composition and phospholipid profiles also confirmed the affiliation of the two strains to the genus Nonomuraea. However, DNA-DNA hybridization data, together with physiological and morphological properties, showed that two isolates could be readily distinguished from their close phylogenetic relatives. Thus, on the basis of these phenotypic and genotypic data, strains NEAU-DE8(1)T and NEAU-HE1(2) are concluded to represent a novel species of the genus Nonomuraea, for which the name Nonomuraea lycopersici sp. nov. is proposed. The type strain is NEAU-DE8(1)T (=CGMCC 4.7412T=DSM 104642T).