Nonlinear optical investigation of normal ovarian cells of animal and cancerous ovarian cells of human in-vitro

Abstract

Laser-based study of two types of ovarian cells has been reported. For both normal and cancerous ovarian cells, growth and reproduction of cells formed to transparent and fresh substrates. Normal and cancerous cells fixed on glass substrate and nonlinear behavior of this microsize biolayer investigated by Z-scan technique using CW He–Ne laser. The magnitude and sign of nonlinear refractive index of normal and cancerous ovarian cells have been reported. Results show that nonlinear optical behavior of normal animal (Wistar rat) and cancerous human cells is different. Nonlinear refractive index (n2) of all studied normal biolayers has negative sign and vice versa for cancerous biolayers. Additionally magnitude of n2 for all samples was in order of 10−8 (cm2/w). So optical apparatus like Z-scan can be used for discriminate normal and cancerous ovarian cells and may be considered for optical diagnostic methods in medicine later.