Abstract
Phorbol 12-myristate 13-acetate (PMA), a tumor promoter, provides effective means of reversibly converting tissue culture cells to a state which resembles that of transformed cells. PKC isozymes can be activated by PMA and cause reorganization of actin-containing structures. The purpose of the current research is to analyze the pattern in which PKC mediates dissolution of stress fibers (SFs) by a mathematical model. Several mathematical models were tried, including linear and nonlinear approximations, in order to establish the relationship between PKC-? and SF accumulation in 1000 W cells. Applying a non-linear approximation and using a bisection algorithm, an exponential function was derived to show how PKC-? inversely controlled SFs. We use RNA interference (RNAi) technology to knock down the PKC-? expression in order to validate this mathematical model. In the time course of SF formation following PMA exposure, a special feature of parallel arrays showed in cells with PKC-? siRNA transfection.
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