Nonlinear microscopy for the visualization of calcification and assessment of connective tissue fibers in pseudoxanthoma elasticum

Abstract

Pseudoxanthoma elasticum (PXE, OMIM 264800) is a rare disorder caused by mutations of the ABCC6 gene in which decreased plasma levels of inorganic pyrophosphate (PPi) lead to ectopic mineralization resulting in severe dermatological, ophthalmologic and cardiovascular complications. Components of the dermal connective tissue can be differentiated with nonlinear optical (NLO)