Abstract
We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).
Highlights
Boldenone and methylbodenone are anabolic steroids often illegally used as growth promoters in animals for human consumption
The resonant behaviour of the double grating waveguide structures (DGWS) was characterized by its transmission spectra
Received 17 Jul 2008; revised 8 Aug 2008; accepted 8 Aug 2008; published 13 Aug 2008 18 August 2008 / Vol 16, No 17 / OPTICS EXPRESS 13318 same 812.7nm wavelength, the resonance occurred at a higher incident angle of 45 ̊, with a full width half maximum (FWHM) of 1.3nm
Summary
Boldenone and methylbodenone are anabolic steroids often illegally used as growth promoters in animals for human consumption. Due to their potential health risk in consumers, the use of natural and synthetic hormones is banned in the European Union (Directive 88/146/EEC). One photon fluorescence (OPF) techniques have shown impressive detection limits [3]. Two photon fluorescence (TPF) techniques exploiting the advantages of nonlinearities have been performed [4,5,6,7]. It was demonstrated that using enhancement platforms such as optical resonators TPF signals were 300-fold enhanced without the need of focusing the laser pulses [6]. The application of DGWS to biosensing has been proposed and demonstrated with OPF as an analytical tool [9, 10]
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