Abstract
Brillouin spectroscopy is an emerging tool in biomedical imaging and sensing. It is capable of assessing the high-frequency viscoelastic longitudinal modulus with microscopic spatial resolution. Nonlinear Brillouin spectroscopy based on impulsive stimulated Brillouin scattering offers a number of significant advantages over conventional spontaneous and stimulated Brillouin scattering. In this report, we evaluate the accuracy of Brillouin shift measurements in spontaneous and nonlinear Brillouin microscopy by calculating the Allan variance for both CW excited spontaneous Brillouin measurements and nonlinear Brillouin scattering measurements made with both nanosecond and picosecond pulse excitation. We find that impulsive stimulated Brillouin spectroscopy is superior to spontaneous Brillouin spectroscopy in terms of the accuracy of such measurements and demonstrate its application for assessing tiny changes in Brillouin frequency shifts associated with low concentrations of biologically relevant solutions.
Highlights
Elastic properties of molecular, sub-cellular and cellular structures play a crucial role in many areas of Biology and Medicine
The overall complexity of the experimental setup required for attaining a high resolution Brillouin spectrum for exact Brillouin shift and linewidth assessment prompted the use of impulsive stimulated Brillouin scattering (ISBS) [27, 28] to overcome these shortcomings [29, 30]
We present a statistic comparison of data taken with ISBS and SpBS
Summary
Sub-cellular and cellular structures play a crucial role in many areas of Biology and Medicine. It would be of great interest to have a performance rubric in order to compare between the two techniques and determine which method is most appropriate for the task at hand This is especially important to establish optimal power and/or pulse intensity (or pulse length) parameters for imaging cells and tissues that are moving or are damaged. After a short theoretical introduction, we provide a brief overview of the experimental configurations used and present the data, collected for different experimental arrangements, paying special attention to the data statistics in order to determine optimal samples needed for minimum uncertainty We compare both spontaneous Brillouin and ISBS spectroscopies in the discussion section and show how ISBS can be used to evaluate viscoelastic properties of solutions with a small variation of chemical composition
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