Abstract
A spectrofluorometric method is described for the determination of germanium(IV) based on its complexation reaction with quercetin in presence of Brij-35. The fluorescence is monitored at 552nm with excitation at 432nm. The pH, quercetin and Brij-35 concentrations were optimized simultaneously using the response surface methodology from sequential experimental three-variable Doehlert designs. Interferences in this determination have been studied. The detection limit is 7.4ngml−1 and the linear range is between 7.4 and 150ngml−1. The method has been applied to the determination of germanium in a drug and in wholemeal oats.
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