Abstract
Investigation of tumor development is essential in cancer research. In the laboratory, living cell culture is a standard bio-technology for studying cellular response under tested conditions to predict in vivo cellular response. In particular, the colony formation assay has become a standard experiment for characterizing the tumor development in vitro. However, quantification of the growth of cell colonies under a microscope is difficult because they are suspended in a three-dimensional environment. Thus, optical coherence tomography (OCT) imaging was develop in this study to monitor the growth of cell colonies. Cancer cell line of Huh 7 was used and the cells were applied on a layer of agarose hydrogel, i.e., a non-adherent surface. Then, cell colonies were gradually formed on the surface. The OCT technique was used to scan the cell colonies every day to obtain quantitative data for describing their growth. The results revealed the average volume increased with time due to the formation of cell colonies day-by-day. Additionally, the distribution of cell colony volume was analyzed to show the detailed information of the growth of the cell colonies. In summary, the OCT provides a non-invasive quantification technique for monitoring the growth of the cell colonies. From the OCT images, objective and precise information is obtained for higher prediction of the in vivo tumor development.
Highlights
To study the development of tumor or evaluate the capability of the malignant cells, animal experiments are generally conducted [1,2,3]
On the other hand, living cell culture is a bio-technology for predicting in vivo cellular responses based on in vitro processing
Cancer cells encapsulated and suspended in soft hydrogel can proliferate and form cell colonies. This technique is referred to colony formation assay that becomes a standard experiment for characterizing the tumor development in vitro [7,8]
Summary
To study the development of tumor or evaluate the capability of the malignant cells, animal experiments are generally conducted [1,2,3]. On the other hand, living cell culture is a bio-technology for predicting in vivo cellular responses based on in vitro processing It is a less expensive technique and with high biological relevance. Cancer cells encapsulated and suspended in soft hydrogel can proliferate and form cell colonies (or called tumor spheroids) This technique is referred to colony formation assay that becomes a standard experiment for characterizing the tumor development in vitro [7,8]. The OCT provides a non-invasive quantification technique for monitoring and characterizing the growth of the cell colonies. From the OCT images, objective and precise information are obtained for higher prediction of the in vivo tumor development
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
