Non-Invasive Diagnosis of Diabetes by Volatile Organic Compounds in Urine Using FAIMS and Fox4000 Electronic Nose

Siavash Esfahani,Alfian Wicaksono,James Covington,Ramesh Arasaradnam,Ella Mozdiak

doi:10.3390/bios8040121

Siavash Esfahani, Alfian Wicaksono + Show 3 more

Open Access

https://doi.org/10.3390/bios8040121

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Abstract

The electronic nose (eNose) is an instrument designed to mimic the human olfactory system. Usage of eNose in medical applications is more popular than ever, due to its low costs and non-invasive nature. The eNose sniffs the gases and vapours that emanate from human waste (urine, breath, and stool) for the diagnosis of variety of diseases. Diabetes mellitus type 2 (DM2) affects 8.3% of adults in the world, with 43% being underdiagnosed, resulting in 4.9 million deaths per year. In this study, we investigated the potential of urinary volatile organic compounds (VOCs) as novel non-invasive diagnostic biomarker for diabetes. In addition, we investigated the influence of sample age on the diagnostic accuracy of urinary VOCs. We analysed 140 urine samples (73 DM2, 67 healthy) with Field-Asymmetric Ion Mobility Spectrometry (FAIMS); a type of eNose; and FOX 4000 (AlphaM.O.S, Toulouse, France). Urine samples were collected at UHCW NHS Trust clinics over 4 years and stored at −80 °C within two hours of collection. Four different classifiers were used for classification, specifically Sparse Logistic Regression, Random Forest, Gaussian Process, and Support Vector on both FAIMS and FOX4000. Both eNoses showed their capability of diagnosing DM2 from controls and the effect of sample age on the discrimination. FAIMS samples were analysed for all samples aged 0–4 years (AUC: 88%, sensitivity: 87%, specificity: 82%) and then sub group samples aged less than a year (AUC (Area Under the Curve): 94%, Sensitivity: 92%, specificity: 100%). FOX4000 samples were analysed for all samples aged 0–4 years (AUC: 85%, sensitivity: 77%, specificity: 85%) and a sub group samples aged less than 18 months: (AUC: 94%, sensitivity: 90%, specificity: 89%). We demonstrated that FAIMS and FOX 4000 eNoses can discriminate DM2 from controls using urinary VOCs. In addition, we showed that urine sample age affects discriminative accuracy.

Highlights

The growing rate of diabetes and its related diseases is becoming a worldwide major health concern
Field-Asymmetric Ion Mobility Spectrometry (FAIMS) samples were analysed for all samples aged 0–4 years (AUC: 88%, sensitivity: 87%, specificity: 82%) and sub group samples aged less than a year (AUC (Area Under the Curve): 94%, Sensitivity: 92%, specificity: 100%)
We demonstrated that FAIMS and FOX 4000 eNoses can discriminate

Summary

Introduction

The growing rate of diabetes and its related diseases is becoming a worldwide major health concern. Using eNose technology with urinary volatile organic compounds. The mimicry of a biological olfactory system, called the electronic nose, was developed in the early. By developing technology and increasing demand for non-invasive methods for diagnosis diseases, the electronic nose is becoming a promising instrument in the medical domain. Commercial and experimental electronic noses have been developed for diagnosis of a wide range of diseases such as lung cancer [4], breast cancer [5], brain cancer [6] and melanoma [7], prostate cancer [8], colorectal cancer [2], asthma [9], and many other diseases. There are only few studies on diagnosing diabetes using urinary VOCs with eNose instruments [10,11]

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