Abstract

Non-interferometric three-dimensional (3D) fluorescence imaging techniques for bio-applications are presented. For bioapplications, the efficient illumination is very important to avoid the damage of living cells. We have proposed a method to obtain the complex amplitude of the fluorescence light wave with partially coherence by using the transport of intensity equation (TIE). We will show some experimental results of the reconstructed fluorescence distributions of fluorescence beads and living plant cells by TIE.

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