Abstract
Single-nucleotide polymorphisms located within CpG islands are found to cause differences in CpG methylation between alleles, reflecting differences in gene expression.
Highlights
Differential DNA methylation between alleles is well established in imprinted genes and the X chromosomes in females but has rarely been reported at non-imprinted loci on autosomes
Genome Biology 2009, Volume 10, Issue 12, Article R138 Zhang et al R138.2 reports about allele-specific methylation (ASM) on autosomes not connected to the parental inheritance of the alleles [8,9,10]
We discovered ASM of three cytosine-guanine dinucleotide (CpG)-rich regions in gene promoters in leukocyte DNA derived from a healthy individual using bisulfite conversion, subcloning and sequencing [12]
Summary
Differential DNA methylation between alleles is well established in imprinted genes and the X chromosomes in females but has rarely been reported at non-imprinted loci on autosomes. Differential DNA methylation between alleles occurs in imprinted genes [4,5] and on the female X chromosomes [6,7]. We discovered ASM of three cytosine-guanine dinucleotide (CpG)-rich regions in gene promoters in leukocyte DNA derived from a healthy individual using bisulfite conversion, subcloning and sequencing [12]. We studied the methylation pattern of 16 CpG-rich regions in gene promoters of chromosome 21 in up to 38 individuals by bisulfite conversion, subcloning and sequencing of individual clones. We checked the inter-individual DNA methylation difference at these gene promoters with respect to a potential link to age and gender differences
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