Abstract
With a view of obtaining an antihuman hemolytic amboceptor on a large scale, I immunized a goat with thoroughly washed human corpuscles. I obtained a serum of a titre of 0.01 cubic centimeter, that is, capable of complete hemolysis with I cubic centimeter of a one per cent, suspension of washed human corpuscles in the presence of 0.01 cubic centimeter of normal goat's or guinea-pig's serum. The reactivating property of normal goat's serum for this ambocepter was found to be somewhat superior to that of guinea-pig's complement. As it would be of great economical value for complement fixation tests to utilize an amboceptor and complement from such a large animal as a goat, instead of using an amboceptor from rabbits and complement from guinea-pigs, I tested the amboceptor and complement from goats as to the possibility of using them in complement fixation tests in general. It was soon found that no complement-fixation phenomenon can be obtained by using them (antihuman amboceptor from this immune goat and c...
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