Abstract
In the present study, a screen-printed electrode based-sensor with electrochemical detection was developed for rapid and sensitive determination of acetylcholine. At first, the screen-printed carbon electrode was modified by using a magnetic core shell and then was used for voltammetric determination of acetylcholine in ampoule, serum and urine samples. The electrochemical behaviour of acetylcholine at the modified electrode was investigated by cyclic voltammetry. The modified electrode displayed a decrease in the overpotential (ca. 130 mV) and an obvious increase in the peak current compared to the non-modified screen-printed electrode. The results indicated that modified screen-printed electrode enhanced electrocatalytic activity towards the oxidation of acetylcholine. Under optimized conditions, the limit of detection from the experiment of acetylcholine determination was 0.02 µM with acetylcholine concentration in range of 0.1–500.0 µM. The reproducibility of the measurements was tested by recording the responses for 50.0 µM acetylcholine with four different developed sensors prepared on the same manner and the same day. A relative standard deviation value of 4.3% was obtained. Finally, a recovery test is done as a part of accuracy evaluation of the system.
Highlights
The nervous system plays an important role in the human body and control body functions by regulating and coordinating their various activities, which is mainly carried out in the form of electrochemical signaling by neurotransmitters [1].Acetylcholine (ACh) is an important neurotransmitter in both peripheral and central nervous systems
Various methods had been reported for ACh detection which includes matrix-assisted laser desorption ionization time-of-flight mass spectrometry [7], high performance liquid chromatography coupled to post-column chemiluminescence detection [8], gas chromatography mass spectrometry [9], capillary zone electrophoresis [10], potentiometry [11], colorimetric detection [12], optical detection [13], chemiluminescence [14, 15], photoelectrochemistry [16] and enzymatic electrochemical biosensors [17]
Electrochemical behavior of acetylcholine at the surface MCSNP/SPE The electrochemical behavior of ACh is dependent on the pH value of the aqueous solution
Summary
The nervous system plays an important role in the human body and control body functions by regulating and coordinating their various activities, which is mainly carried out in the form of electrochemical signaling by neurotransmitters [1].Acetylcholine (ACh) is an important neurotransmitter in both peripheral and central nervous systems. PH optimization of the solution seems to be necessary in order to obtain the best results for electrooxidation of ACh. the electrochemical behaviors of ACh were studied in 0.1 M PBS in different pH values (2.0–9.0) at the surface of MCSNP/SPE by voltammetry.
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