Abstract
This study aimed to determine the type of seed dormancy and to identify a suitable method of dormancy-breaking for an efficient seed viability test of Lysimachia coreana Nakai. To confirm the effect of gibberellic acid (GA3) on seed germination at different temperatures, germination tests were conducted at 5, 15, 20, 25, 20/10, and 25/15 °C (12/12 h, light/dark), using 1% agar with 100, 250, and 500 mg·L−1 GA3. Seeds were also stratified at 5 and 25/15 °C for 6 and 9 weeks, respectively, and then germinated at the same temperature. Seeds treated with GA3 demonstrated an increased germination rate (GR) at all temperatures except 5 °C. The highest GR was 82.0% at 25/15 °C and 250 mg·L−1 GA3 (4.8 times higher than the control (14.0%)). Additionally, GR increased after cold stratification, whereas seeds did not germinate after warm stratification at all temperatures. After cold stratification, the highest GR was 56.0% at 25/15 °C, which was lower than the GR observed after GA3 treatment. We hypothesized that L. coreana seeds have a non-deep physiological dormancy and concluded that 250 mg·L−1 GA3 treatment is more effective than cold stratification (9 weeks) for L. coreana seed-dormancy-breaking.
Highlights
The dried seeds were sealed in a glass bottle and stored in a seed bank at −20 ◦ C and 40% humidity until they were required for the experiments
Treatments were 82 and 56%, respectively, with a low to achieve a 50% germination rate (T50) (Figure 3). These results indicate that the L. coreana seeds break dormancy through GA3 treatment or cold stratification (CS), but treatment with
Coreana may be categorized as a non-deep physiological dormancy (PD) type, and GA3 or CS at 25/15 ◦ C is required to break dormancy and promote germination
Summary
Received: 19 October 2021Accepted: 11 November 2021Published: 12 November 2021Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations.Licensee MDPI, Basel, Switzerland.Attribution (CC BY) license (https://creativecommons.org/licenses/by/ 4.0/).Global climatic change due to increasing greenhouse gas emissions has induced drought and/or higher temperatures in recent decades [1]. Seed germination and seedling establishment are both influenced by rising temperature and moisture limitation, making these life stages particularly sensitive to climate change [1,2,3,4,5]. Most species of the genus
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