Abstract
Clostridium ihumii strain AP5T sp. nov. is a new species within the genus Clostridium. This strain, whose genome is described here, was isolated from the stool sample of a 21-year-old French Caucasian female with anorexia nervosa. C. ihumii is a Gram-positive, anaerobic bacillus. Here we describe the features of this organism, together with the complete genome sequence and annotation. The 4,433,668 bp long genome contains 4,076 protein-coding and 85 RNA genes, including 9 rRNA genes.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0025-x) contains supplementary material, which is available to authorized users.
Highlights
Clostridium ihumii strain AP5T (=CSUR P198 = DSM 26098) is the type strain of C. ihumii sp. nov. This bacterium is a Gram-positive, anaerobic rod-shaped bacteria that was isolated from the stool sample of 21-year-old French Caucasian female with anorexia nervosa since the age of 12 years, as a part of a “culturomics” study aiming at cultivating all species within human feces individually [1,2,3]
Using a large scale of culture conditions, MALDI-TOF MS and 16S ribosomal RNA sequencing, C. ihumii and 10 new bacterial species have been successfully identified in a single stool sample [4, 5]
On the basis of phenotypic, phylogenetic and genomic analyses, we formally propose the creation of Clostridium ihumii sp. nov. that contains strain AP5T
Summary
Genome project history The organism was selected for sequencing on the basis of its phylogenetic position and 16S rRNA similarity to members of the genus Clostridium and is part of a study of the human digestive flora aiming at isolating all bacterial species within human feces [1]. It was the 102nd genome from the genus Clostridium and the first genome of C. ihumii sp. The draft genome sequence of C. ihumii strain AP5T is smaller than those of C. beijerinckii and C. difficile (6.0 and 4.46 Mb, respectively), but larger than those of C. carboxidivorans, C. botulinum, C. senegalense, C. dakarense, C. perfringens, and (4.41, 3.9, 3.89, 3.73 and 3.26 Mb, respectively). When C. ihumii was compared to other species, AGIOS values ranged from 70.85 with C. beijerinckii to 79.10% with C. senegalense (Table 5B)
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