Non-contact quantitative diffuse reflectance spectroscopy

Abstract

We validate a non-contact Diffuse Reflectance Spectroscopy (DRS) system as a first stage to approach quantitative multi-spectral imaging technique. The non-contact DRS system with separated illumination and detection paths was developed with different progressive set-ups which were all compared to a well-founded contact DRS system. While quantitation of the absorption coefficient is well achieved with the existing method, the calculation of the scattering coefficient is deteriorated by the non-contact architecture measurements. We have therefore developed an adaptive reference-based algorithm to compensate for this effect.