Abstract
Antinutritional factors, viz. glucosinolates, phenols, and phytic acid, decreased with germination of rapeseed. Protein content declined during the first 24 h after imbibition and thereafter increased. Phytase activity increased with germination with a parallel decrease in phytic acid content. Two phytase fractions were separated during elution on DEAE-cellulose column, showing purifications of 26.75- and 19.96-fold, respectively. Optimum pH and temperature for both fractions were 5.2 and 50 °C, respectively. Enzyme stability decreased considerably after 50 °C, and phytase 2 was stable for a longer time. Mn, Co, Ca, and EDTA promoted the enzyme activity, whereas ions of Zn, Fe, Cu, fluoride, molybdate, and arsenate were inhibitory. Keywords: Antinutritional factors; characterization; germination; phytase
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