Abstract
A non-biotin amplification (NBA) detection system was compared to current, widely used biotin-labeled secondary antibody combined with streptavidin enzyme conjugate (Lab-SA) detection system. Series of normal and abnormal tissue sections were analyzed with these 2 systems under identical experimental conditions. NBA detection system had similar sensitivity to Lab-SA kits in all 6 cases tested with 6 different primary antibodies at 3 different dilutions. There was no significant difference in staining intensity of the target antigen. Results of multinormal tissue blocks staining with both Lab-SA and NBA detection systenls after heat-induced epitope retrieval (HIER) indicated that HIER reactivated endogenous biotin, and that Lab-SA required an additional step of biotin-blocking for optimal staining. NBA detection system showed no evidence of nonspecific biotin staining caused by endogenous biotin that was induced by HIER procedure in the formalin fixed, paraffin embedded tissues. (The J Histotechnol 23:327, 2000)
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