Nonadherent Zwitterionic Composite Nanofibrous Membrane with a Halloysite Nanocarrier for Sustained Wound Anti-Infection and Cutaneous Regeneration.

Abstract

Wound dressing synechia and sustained postoperative bacterial infection would cause serious secondary damage to nascent cutaneous tissue and impede normal regeneration of injured wound. Endowing wound dressings with nonadherent capability and long-lasting antibacterial property could optimize the postoperative wound healing conditions and promote wound tissue neogenesis, which have important clinical application value and demand. In this study, novel nanocarrier-embedded zwitterionic composite nanofibrous membranes are fabricated using the co-electrospinning/photo-cross-linking method for the purpose of painless removal and eliminating long-lasting antibacterial infection during postoperative wound therapy. The prepared membranes possess good biocompatibility, excellent antibiofouling ability against both bacteria and plasma proteins, and platelet and L929 cell adhesion. Furthermore, in vitro and in vivo antibacterial evaluations exhibit that the composite nanofibrous membranes with a sustained drug release profile could effectively inhibit bacterial proliferation for at least 16 days. Additionally, in vivo wound regeneration assessment indicates that the obtained membranes could better enhance skin regeneration than the commercial 3M Tegaderm film, which highlights the application prospect of such novel zwitterionic composite nanofibrous membranes for sustained postoperative wound anti-infection and cutaneous regeneration.