Non-Specific Immune Potentiating activity of Multivitamins in Catfish (Clarias sp.)

Cahyono Purbomartono,Dini Siswani Mulia,Arief Husin,Ulfatun Rizkiyah

doi:10.30595/sainteks.v18i2.13113

Cahyono Purbomartono, Dini Siswani Mulia + Show 2 more

Open Access

https://doi.org/10.30595/sainteks.v18i2.13113

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Journal: Sainteks	Publication Date: Feb 12, 2022
License type: CC BY 4.0

Affiliation: Muhammadiyah University Purwokerto

Abstract

Fish farming is well developed because many people like to consume fish. The main problem in cultivation is an attack of a disease. Generally, the disease in fish is MAS (Motile Aeromonas Septicemia) which is caused by Aeromonas hydrophila. Prevention of diseases in fish can be done by increasing immunity through the addition of multivitamins in feed. This study aims to determine the ability of multivitamins against non-specific immune responses in African catfish (C. gariepinus) as well as to know the optimum dose. The study used an experimental method with a completely randomized design using 3 treatments, 1 control, and 4 individual replications. The treatment using a multivitamin through feed with a dose of T1 = 2 g kg-1, T2 = 2.5 g kg-1, T3 = 3 g kg-1 and T0 = control. The parameters observed were phagocytic activity, and differential leukocytes of monocytes and lymphocytes. Data were analyzed using Analysis of variance (ANOVA) at the 95% confidence level. If there is a real difference, then proceed with the Duncan Multiple Range test Test (DMRT) at a 95% confidence level. The results showed that the addition of multivitamins through feed on African catfish had a significant effect (p<0.05) on phagocytic activity and differential leukocyte of monocytes, but had no significant effect (p> 0.05) on differential leucocytes of lymphocyte. The optimum dose for increasing non-specific immune responses in African catfish is T2 at a dose of 2.5 g kg-1 of feed. Based on research, the addition of multivitamins through the feed can potentially be used in fish farming to increase immunity.

Highlights

African catfish (Clarias gariepinus) are freshwater fish that have been commercially cultivated in Indonesia
The cultivation of African catfish is growing rapidly because it has prospects of high economic value (Bachtiar, 2006). This is proven by the increasing demand in the market so it has an impact on development in Indonesia
The results showed the addition of a multivitamin through the feed can increase the immunity in catfish

Summary

INTRODUCTION

African catfish (Clarias gariepinus) are freshwater fish that have been commercially cultivated in Indonesia. The cultivation of African catfish is growing rapidly because it has prospects of high economic value (Bachtiar, 2006) This is proven by the increasing demand in the market so it has an impact on development in Indonesia. Rawung & Manoppo (2014), said that immunostimulants are added to feed can increase fish resistance to disease infections through increasing non-specific immunity. The addition of multivitamins in this study was based on the various roles of multivitamins in increasing fish immunity and growth. Among these multivitamins are vitamin B complex, C, E, and A (Sakai, 1999). The addition of vitamin C in the diet can increase the non-specific immune response of the mud grouper, Epinephelus coioides (Johnny et al, 2007)

RESEARCH METHOD

AND DISCUSSION

Findings

10 T1 T2 T3 day-0