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Abstract
Iron is an essential nutrient for most living bacteria. One of the main mechanisms which allow bacterial pathogens to obtain iron is siderophore-mediated iron acquisition. Aeromonas salmonicida subsp. salmonicida, the aetiological agent of furunculosis in fish produces a catecholate siderophore under iron-limiting conditions. A. salmonicida siderophore biosynthesis genes include genes that encode non-ribosomal peptide synthetases. In this study using a siderophore biosynthesis gene cluster previously described, the asbD gene which encodes a non-ribosomal peptide synthetase was studied. Total protein of null mutant and wild-type strains, in iron-repleted and iron-depleted conditions, was extracted and loaded onto polyacrylamide gel. Only the wild-type strain in iron-depleted conditions expressed a 160-KDa protein. These results confirmed that asbD is an iron-regulated gene, and AsbD protein is expressed only in iron-limited conditions.
Published Version
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