Abstract
Summary The objective of this study was to validate a gill biopsy method for non-lethal monitoring of the osmoregulatory status of young sturgeon in estuary areas. After gill biopsy, hybrid sturgeon (Acipenser ruthenus ♀ 9 A. schrenckii ♂) were transferred from freshwater to brackish water (10 ppt) for 6 weeks. Mortality, serum osmolality, ion concentrations and gill Na + ,K + -ATPase activity of fish exposed to brack
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