Abstract
We characterized the endothelin (ET) receptor in Girardi heart (GH) cells derived from human atrium. The ET isopeptides ET-1, ET-2 and ET-3 induced the monotonous and long-lasting rise in cytosolic free Ca 2+ concentration ([Ca 2+] i) with almost the same potency in GH cells. Scatchard analysis of [ 125I]ET-1 and [ 125I]ET-3 binding revealed that GH cells have almost the same number of binding sites for either labeled ligand. All ET isopeptides displaced either [ 125I]ET-1 or [ 125I]ET-3 binding in GH cells almost equipotently. These results reveal that the functional ET receptors in GH cells are of the ET B-type. GH cells are the first cell line to be found to express the functional ET B-receptor.
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