Non-invasive diagnostic PCRs for rapid detection of golden jackal, red fox, and gray wolf/domestic dog and application to validate golden jackal presence in Styria, Austria

Abstract

Human-predator conflicts are frequently caused by livestock and/or game depredation. The golden jackal’s (Canis aureus) range expansion in Europe, as well as the recent re-expansion of several gray wolf (Canis lupus) populations, might increase risk of such conflicts. In Austria, golden jackal presence has been increasing since the 1990s including reports of wildlife and livestock kills, frequently occurring in the provinces Styria and Burgenland. We developed a rapid, two-step genetic screening protocol to (1) detect canid mtDNA from non-invasively collected samples like swabs from kills using diagnostic PCRs, and (2) assign this DNA to red fox (Vulpes vulpes), golden jackal, or gray wolf/dog. To monitor golden jackal presence in the region, a total of 167 signs of presence were collected over a period of 30 months throughout the Styrian province. Among these, 14 non-invasive genetic samples (13 swabs from kill sites and one scat) were screened with the developed protocol. Four of these samples revealed golden jackal mtDNA and six samples red fox mtDNA. The developed genetic screening protocol represents a quick and inexpensive method to assess canid presence, e.g., at kill sites, and therefore possesses high value for the conservation and wildlife management community.