Non-immersed zigzag microalgae biofilm overcoming high turbidity and ammonia of wastewater for muti-pollutants bio-purification

Abstract

Biological treatment that utilizes microalgae technology has demonstrated outstanding efficacy in the wastewater purification and nutrients recovery. However, the high turbidity of the digested piggery wastewater (DPW) leads to serious light attenuation and the culture mode of suspended microalgae results in a huge landing area. Thus, to overcome light attenuation in DPW, a non-immersed titled zigzag microalgae biofilm was constructed by attaching it onto a porous cotton cloth. As a result, the light could directly irradiate microalgae biofilm that attached on both sides of the cotton cloth, and the microalgal biofilm area was up to 6 m2 per bioreactor landing area. When the non-immersed zigzag microalgae biofilm bioreactor (N-Z-MBP) was used to treat wastewater with an ammonia nitrogen (NH4+-N) concentration of 362 mg L−1, the NH4+-N was completely removed in just 5 days and the maximum growth rate of microalgae biofilm reached 7.02 g m−2 d−1. After 21 days of long-term sequencing batch operation for the N-Z-MBP, the biomass density of the biofilm reached 52 g m−2 and remained at this high value for the next 14 days. Most importantly, during the 35 days’ running, the NH4+ -N maximum removal rate of single batch reached up to 65 mg L−1 d−1 and its concentration in the effluent was always below the discharge standard value (80 mg L−1 form GB18596–2001 of China) and total phosphorus was completely removed in each batch. Furthermore, the biomass concentration of microalgae cells in the effluent of the N-Z-MBP was almost zero, indicating that the non-submerged biofilm achieved in situ separation of microalgae from the wastewater. This work suggests that the N-Z-MBP can effectively purify DPW over a long period, providing a possible strategy to treat wastewater with high ammonia nitrogen and high turbidity.