Non-enzymatic simultaneous detection of acetylcholine and ascorbic acid using ZnO·CuO nanoleaves: Real sample analysis

Abstract

A facile wet-chemical technique used to prepare the zinc oxide doped copper oxide nanoleaves (ZnO·CuO nanoleaves) in alkaline phase. ZnO·CuO nanoleaves were examined using a variety of conventional techniques for example UV–Visible, FTIR, XRD, FESEM equipped XEDS, and XPS. A non-enzymatic sensor was prepared with modification of a slightly deposited of ZnO·CuO nanoleaves onto a flat GCE, which fabricated through a conducting nafion polymer matrix in order to detect selective acetylcholine and ascorbic acid simultaneously. Analytical performances such as sensitivity, LOD, LOQ, LDR, and durability of the selective acetylcholine and ascorbic acid sensors were examined through a conventional current–voltage method. Calibration graphs of acetylcholine and ascorbic acid sensors were found linear (R2 = 0.9049 and 0.9201) over a good concentration range (100.0 pM–100.0 mM) respectively. Sensitivity (317.0 and 94.94 pAμM-1cm−2), LOD (14.7 and 12.0 pM), and LOQ (490.0 and 367.0 mM) of acetylcholine and ascorbic acid sensors were calculated correspondingly from the slope of the linear portion of calibration graph. Preparation of ZnO·CuO nanoleaves using wet-chemical technique is an excellent approach for the advancement of nanomaterial based on sensor development in support of enzyme free detection of biological molecules for the safety in healthcare and biomedical fields. This expected sensor applied for the specific determination of acetylcholine and ascorbic acid in real samples (Human, mouse, and rabbit serum, orange juice, and urine) and found satisfactory and acceptable results.